ISOLATION AND STRUCTURE ELUCIDATION OF ERYTHRIN AND BIOPROSPECTION STUDIES OF ROCCELLA MONTAGNEI EXTRACT

Objectives: Lichen is a stable mutually beneficial organism. Composed of fungi, Mycobiont united with algae, phycobiont or cyanobacteria. Since the ancient period, extract derived from Lichens utilized in medication numerous diseases. An attempt was made to isolate and structure elucidation erythrin, biologically active compound corticolous lichen Roccella montagnei, analyze antimicrobial antioxidant activities lichen’s acetone under vitro conditions. Methods: characterized R. montagnei using ultraviolet, Fourier transform infrared, High-performance liquid chromatography, nuclear magnetic resonance, gas chromatography–mass spectrometry techniques. The were evaluated against six pathogenic microorganisms standard disc diffusion technique. For activity, 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) scavenging method, hydrogen peroxide phosphomolybdenum assay done. Results: outcomes show that has maximum effective antibacterial activity compared antifungal microorganisms. zone inhibition recorded Escherichia coli (8.75±0.61 mm). Among fungal pathogens, inhibited growth Candida albicans 7.50±0.52 mm. In DPPH IC50 value extract, ascorbic acid found be 45.70 μg mL-1 39.74 mL-1, respectively. it observed acid, rutin 39.39 40.66 45.58 content 44.66 mg/g equivalents 100 assay. Total phenolic higher 214.84±14.84 mg gallic equivalent (GAE/g extract). Conclusion: present study did isolation erythrin obtained Anaikatty reveals potential control human future also suggested possesses substances scavenge free radicals.